---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-09-19 04:44:12+00:00
layout: post
slug: mrna-isolation-gigas-bb-and-dh-samples-previously-treated-with-ribominus-kit-by-mac
title: mRNA Isolation - Gigas BB and DH samples previously treated with Ribominus
  Kit (by Mac)
categories:
  - 2009
  - Miscellaneous
tags:
  - BB
  - Crassostrea gigas
  - DH
  - EtOH precipitation
  - Micro PolyA Purist
  - mRNA
  - mRNA enrichment
  - Pacific oyster
  - RNA
---

Was given ~0.5ug of each of these two RNA samples and processed them with Ambion's microPolyA Purist Kit according to protocol. After elution, the samples were EtOH precipitated @ -80C for 30mins, pelleted 30mins 16,000g for 30mins, 4C. Supe removed, RNA washed with 1mL 70% EtOH and spun 10mins 16,000g, 4C. Supe removed. Resusupended in 10uL of The RNA Storage Solution and gave back to Mac.