---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-09-17 04:45:58+00:00
layout: post
slug: mrna-isolation-gigas-bb-and-dh-samples-previously-treated-with-ribominus-kit-by-mac-2
title: mRNA Isolation - Gigas BB and DH samples previously treated with Ribominus
  Kit (by Mac)
categories:
  - 2009
  - Miscellaneous
tags:
  - BB
  - Crassostrea gigas
  - DH
  - EtOH precipitation
  - mRNA
  - mRNA enrichment
  - NanoDrop1000
  - Pacific oyster
  - PolyA Tract Kit
  - RNA
  - RNA quantification
---

Was given 1ug of each of these two RNA samples and processed them with Promega's PolyA Tract Kit according to protocol. After elution, the samples were EtOH precipitated @ -20C for 30mins, pelleted 30mins 16,000g for 30mins, 4C. Supe removed, RNA washed with 1mL 70% EtOH and spun 15mins 16,000g, 4C. Supe removed. Resusupended in 15uL of 0.1% DEPC-H2O and spec'd.

Results: No measurable amount of RNA in either sample. Samples were stored @ -80C.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090916%20mRNA%20BB%20DH%20ribominus_polyA.jpg)