---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-08-27 15:56:58+00:00
layout: post
slug: qpcr-gigas-gdna-test-of-recalibrated-opticon-2
title: qPCR - Gigas gDNA test of recalibrated Opticon 2
categories:
  - 2009
  - Miscellaneous
tags:
  - calibration
  - Crassostrea gigas
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO 13
  - troubleshooting
---

Master mix containing Gigas gDNA will be used to verify that the recalibration did work. [qPCR setup/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090827-01.jpg). I've made a master mix using Cg_HSP70_F/R primers designed by Mac. gDNA is BB #12 (0.445ug/uL) from [20090519](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090519). The gDNA will be added to the master mix.

Results: The results are a bit disconcerting, as this run shows virtually the exact same pattern in fluorescence detection as that on [20090722](/Sam%27s+Working+Notebook+Jun-Aug+2009#sjw20090722), despite using a different set of gigas gDNA. Below is a set of graphs comparing Column 1 Ct values of the two tests from [20090722](/Sam%27s+Working+Notebook+Jun-Aug+2009#sjw20090722) and today:

![](https://img.skitch.com/20090827-8rwrr6161dc2gt74k1gj19tspp.jpg)

Clearly, both runs exhibit virtually the same pattern of relative Ct values to each other in each respective run. Not cool.