---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-08-24 16:10:32+00:00
layout: post
slug: qpcr-additional-calibration-test-of-opticon-2
title: qPCR - Additional Calibration test of Opticon 2
categories:
  - 2009
  - Miscellaneous
tags:
  - calibration
  - Opticon2
  - qPCR
  - troubleshooting
---

Based on recs from Bio-Rad rep (Carl Fisher), will repeat Opticon 2 calibration (see [20090813](/Sam%27s+Working+Notebook+Jun-Aug+2009#sjw20090813)) test according to Opticon manual. Then, will rotate plate 180 degrees and repeat test and upload data to Bio-Rad server for analysis and evaluation.

Results: According to the Bio-Rad rep, he thinks recalibration is in order. He pointed out that we're seeing a 3-fold spread in fluorescence, which is outside of the "tolerable" range (which is 2-fold). Will recalibrate.
