---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-23 16:15:54+00:00
layout: post
slug: bacteria-c-pugetti-culture-from-20090713
title: Bacteria - C.pugetti culture (from 20090713)
categories:
  - 2009
  - Miscellaneous
tags:
  - bacterial culture
  - Cycloclasticus pugetii
  - JGI
---

1L liquid culture (grown for 10 days) was split into two UV-sterilized bottles. Cells were pelleted in a Sorvall T21 centrifuge using the bucket rotor for 30mins @ 4200RPM, 4C. Supe was removed and bacterial pellets were stored @ -20C.