---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-17 16:22:15+00:00
layout: post
slug: qpcr-abalone-gdna-h-crach-067-1
title: qPCR - Abalone gDNA (H.crach 06:7-1)
categories:
  - 2009
  - Miscellaneous
tags:
  - '06:7-1'
  - black abalone
  - gDNA
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO 13
---

This is to test (again!) the H.crach_h-1fg_intron primers and obtain a working positive control. This gDNA is from Lisa/Nate. Acquired from them 20090717. Don't know date/method of isolation, but this should be good, recent gDNA. [qPCR plate layout/set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090717-01.jpg). Anneal temp 50C. Ran serial dilutions of the gDNA: undiluted, 1:10 and 1:100.

Results: Signals look good. The undiluted comes up around 30 cycles. Should be able to use it as a positive control for gDNA detection PCRs.