---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-17 02:35:10+00:00
layout: post
slug: qpcr-abalone-cdna-0712-set-from-332009-by-lisa-and-dnased-rna-from-20090623-2
title: qPCR - Abalone cDNA (07:12 set from 3/3/2009 by Lisa) and DNased RNA (from
  20090623)
categories:
  - 2009
  - Miscellaneous
tags:
  - '07:12'
  - black abalone
  - cDNA
  - DNased RNA
  - graphs
  - H.crach_h-1fg_intron
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

This is being done to check whether or not there is gDNA contamination in these cDNA and DNased RNA. Will use H.crach_h-1fg_intron primers. gDNA 07:12-15 was used as a positive control, based on results from yesterday's qPCR. [qPCR plate layout/set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090716-01.jpg). Anneal temp 50C.

Results: Everything came up negative, including the positive control! Also, the machine experienced an error at ~cycle 39, so no melting curve info. See below.

![](https://eagle.fish.washington.edu/Arabidopsis/20090716-01%20SJW.jpg)