---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-13 16:51:03+00:00
layout: post
slug: pcr-dungan-isolate-mie-14v-gdna-from-20090708
title: PCR - Dungan isolate (MIE-14v) gDNA from 20090708
categories:
  - 2009
  - Miscellaneous
tags:
  - Dungan
  - Euk
  - gel
  - LABY
  - MIE-14v
  - PCR
---

PCR of MIE-14v just to make sure that we can't get a product from this sample, despite NanoDrop readings suggesting that there's no DNA. Used both LABY and Euk primer sets. [PCR set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090713-02.jpg). Anneal temp 50C.

![](https://eagle.fish.washington.edu/Arabidopsis/20090714.JPG)

Lane 1 - 100bp Ladder

Lane 2 - Euk

Lane 3 - Euk H2O

Lane 4 - Euk H2O

Lane 5 - Euk H2O

Lane 6 - LABY

Lane 7 - LABY H2O

Lane 8 - LABY H2O

Lane 9 - LABY H2O

Lane 10 - 100bp Ladder

Results: Nothing, as expected. Need to devise a new method of isolating gDNA from these "problem" isolates.
