---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-11 16:49:11+00:00
layout: post
slug: qpcr-abalone-gdnacdna
title: qPCR - Abalone gDNA/cDNA
categories:
  - 2009
  - Miscellaneous
tags:
  - black abalone
  - cDNA
  - gDNA
  - H.crach_h-1fg_intron
  - H.iris_actin_intron
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

Due to lack of amplification in gDNA samples from [20090710](/Sam%27s+Working+Notebook+Jun-Aug+2009#sjw20090710) and [20090708](/Sam%27s+Working+Notebook+Jun-Aug+2009#sjw20090708) with either set of intron primers, will repeat with additional gDNA samples to make sure the primers are the problem and not the gDNA. Used the H.iris_actin_intron_Fw/Rv and the H.crach_h-1fg_intron_Fw/Rv primers. [PCR setup/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090713-01.jpg). Anneal temp 50C.

Results: Got a weak signal (C(t) ~ 37) in only the 06:50-9 rxns, but it did work with both primer sets.