---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-08 22:22:58+00:00
layout: post
slug: qpcr-dnased-abalone-dg-rna-from-20090625-2
title: qPCR - DNased Abalone Dg RNA from 20090625
categories:
  - 2009
  - Miscellaneous
tags:
  - '06:50-10'
  - '07:12'
  - black abalone
  - cDNA
  - DNased RNA
  - gDNA
  - H.crach_h-1fg_intron
  - H.iris_actin_intron
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

Ran qPCR on DNased Abalone Dg RNA (07:12 Set), gDNA (06:50-10) and clean cDNA (from [20090422](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090422)) using primers (H.crach_h-1fg_intron_Fw/Rv) designed to bind only to a region in an intron of the H.cracherodii hemocyanin gene. [PCR setup/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090708-01.jpg). Anneal temp of 50C was used.

Results: No PCR products in any samples, not even the positive control. It seems that the primers don't work. Will design new primers, probably from a different species of abalone since there was essentially only one gene in H.cracherodii that had any intron sequence available..