---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-07 22:32:52+00:00
layout: post
slug: qpcr-mv-hemocyte-cdna-from-20090614
title: qPCR - MV hemocyte cDNA from 20090614
categories:
  - 2009
  - Miscellaneous
tags:
  - 18s
  - cDNA
  - Crassostrea virginica
  - Cv_18s_F/R
  - Eastern oyster
  - hemocyte
  - Immomix
  - Martha's Vineyard
  - Opticon2
  - qPCR
  - SYTO13
---

Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:

3326A13

2100B07

3219A06

2100B15

3326A11

2100B12

2100A03

[Plate layout/PCR set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090706-01.jpg). This is a second rep of these samples.

Results: Waters are clean. However, the following samples still are negative for an 18s amplicon: