---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-07-06 22:41:18+00:00
layout: post
slug: spec-reading-c-pugetti-gdna-from-20090526
title: Spec Reading - C.pugetti gDNA from 20090526
categories:
  - 2009
  - Miscellaneous
tags:
  - Cycloclasticus pugetii
  - DNA Quantification
  - gDNA
  - JGI
  - NanoDrop1000
---

A recent email from JGI indicates that they are satisfied with the quality of DNA (as seen on [20090601](/Sam%27s+Working+Notebook+Jun-Aug+2009#sjw20090601)), however their estimate of the gDNA concentration (42ng/uL) means that we have ~16ug of DNA. They requested 50ug. Based on the gel, their calculations are reasonable. However, the NanoDrop suggests that are sample is ~1350ng/uL! So, I've respec'd the sample and did a few dilutions to see how it looked.

![](https://eagle.fish.washington.edu/Arabidopsis/20090706%20DNA%20SJW.jpg)

Results: The undiluted sample is approximately the same concentration as initially reported. A 1:1 dilution produces the expected concentration of half the undiluted. The 1:10 and 1:100 dilutions deviate a bit from the expected concentrations, but are reasonably close. I still don't know how to explain the discrepancy between what the gel analysis suggests vs. the NanoDrop spectrophotometric data.