---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-26 23:03:49+00:00
layout: post
slug: qpcr-mv-hemocyte-cdna-from-20090614-7
title: qPCR - MV hemocyte cDNA from 20090614
categories:
  - 2009
  - Miscellaneous
tags:
  - 18s
  - cDNA
  - Crassostrea virginica
  - Cv_18s_F/R
  - Eastern oyster
  - hemocyte
  - Immomix
  - Martha's Vineyard
  - Opticon2
  - qPCR
  - SYTO13
---

Set up qPCR with Cv_18s_F/R primers. [Plate layout/PCR set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090626-01.jpg). This is a second rep. Used 1:20 cDNA plate made by Mac as template.

Results: Waters are clean. Still some samples that aren't coming up. Will eventually run those samples using undiluted cDNA. To be analyzed later with other genes that Mac has run.
