---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-25 23:27:16+00:00
layout: post
slug: qpcr-abalone-dg-dnased-rna-from-yesterday-and-earlier-today
title: qPCR - Abalone Dg DNased RNA from yesterday and earlier today
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - Dg
  - digestive gland
  - DNased RNA
  - H.crach_16s_SYB_F/R
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - RNA
  - SYTO13
---

Check Abalone RNA for residual gDNA contamination after DNase treatment. Ran qPCR with H.crach_16s_SYB_F/R primers. [Plate layout/PCR set up here.](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090625-01.jpg)

Results: Still gDNA in virtually every sample! This is totally insane. Will find/design primers that will only amplify gDNA to aid in subsequent analysis...
