---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-16 00:37:17+00:00
layout: post
slug: qpcr-mv-hemocyte-cdna-test-immomix-syto13-vs-strategene-sybr
title: 'qPCR - MV hemocyte cDNA: Test Immomix (SYTO13) vs. Strategene SYBR'
categories:
  - 2009
  - Miscellaneous
tags:
  - 18s
  - cDNA
  - Crassostrea virginica
  - Cv_18s_F/R
  - Eastern oyster
  - hemocyte
  - Immomix
  - Martha's Vineyard
  - Opticon2
  - qPCR
  - SYTO13
---

Due to craziness seen in melting curves, fluorescence, and empty wells from the previous run, will compare SYTO vs. SYBR with select MV cDNAs. Additionally, acquired some qPCR strip caps to use instead of the ABI film. Used Cv_18s_F/R primers. [qPCR set up/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090615-03.jpg).

Results: Both seem to work fine. H2O fluorescence is weird, but doesn't come up in the melting curves. Strategene SYBR provides a brighter signal, but results in a higher melting temp than the SYTO.
