---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-13 00:46:34+00:00
layout: post
slug: qpcr-dnased-mv-hemocyte-rna-from-earlier-today-and-turbo-kit-test
title: qPCR - DNased MV hemocyte RNA from earlier today AND Turbo kit test
categories:
  - 2009
  - Miscellaneous
tags:
  - 18s
  - Crassostrea virginica
  - Cv_18s_F/R
  - DNased RNA
  - Eastern oyster
  - Gigas_18s_F/R
  - hemocyte
  - Immomix
  - Martha's Vineyard
  - Opticon2
  - qPCR
  - SYTO13
---

[qPCR set up/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090612-01.jpg). Used Cv_18s_F/R primers for the MV hemocyte RNA and Gigas_18s_F/R primers for the Turbo kit test. Anneal 55C.

Results: DNase treatment worked on all but the following samples: B23, B14, A21. However, these three samples were slightly below the initial, background fluorescence in each sample. The Turbo kit test indicates that all three kits are working perfectly and all can/should be used with confidence for treating samples.
