---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-13 00:44:57+00:00
layout: post
slug: dnase-treatment-mv-hemocyte-rna-from-yesterday
title: DNase Treatment - MV hemocyte RNA from yesterday
categories:
  - 2009
  - Miscellaneous
tags:
  - Crassostrea virginica
  - DNase
  - Eastern oyster
  - hemocyte
  - Martha's Vineyard
  - RNA
  - Turbo DNA-free
---

Samples 3326: B23, A25, A22, B14, A21, A10 B22 came up positive for gDNA still. These were retreated according to Ambion protocol with a brand new Turbo DNA-free DNase kit. Additionally, I tested all three existing kits by "spiking" 19uL of H2O with 1uL (~200ng) of gigas gDNA; one tube for each kit and an untreated sample. Will qPCR to see if gDNA removal was successful.
