--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-06-12 00:49:19+00:00 layout: post slug: rna-isolation-marthas-vineyard-mv-hemocytes title: RNA Isolation - Martha's Vineyard (MV) hemocytes categories: - 2009 - Miscellaneous tags: - Crassostrea virginica - DEPC-H2O - Eastern oyster - Martha's Vineyard - RNA isolation - TriReagent --- Samples were pelleted at 100g, 4C for 10mins. The supe was mostly removed, leaving ~50uL of supe above the pellets. RNA was isolated according to the TriReagent protocol from the following samples: 3226: * A7 * A10 * A15 * A17 * A20 * A22 * A26 * 3326: * B1 * B8 * B10 * B13 * B14 * B22 * B26 * B29 RNAs were resuspended in 10uL of 0.1% DEPC-H2O. They will be DNase treated.