--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-06-12 00:51:53+00:00 layout: post slug: dnase-treatment-mv-hemocyte-rna-from-earlier-today title: DNase Treatment - MV hemocyte RNA from earlier today categories: - 2009 - Miscellaneous tags: - Crassostrea virginica - DNase - Eastern oyster - hemocyte - Martha's Vineyard - NanoDrop1000 - RNA - Turbo DNA-free --- The entire 20uL of RNA were treated with Ambion's Turbo DNA-free kit according to protocol and spec'd. ![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090611%20DNased%20RNA%20SJW.jpg) Mac performed qPCR on the DNase-treated RNAs to verify removal of residual gDNA. Results: Samples 3326: B23, A25, A22, B14, A21, A10 B22 came up positive for residual gDNA. Will retreat these samples and qPCR again.