---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-11 01:47:53+00:00
layout: post
slug: qpcr-re-dnased-abalone-dg-rna-from-earlier-today-2
title: qPCR - Re-DNased abalone Dg RNA from earlier today
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - Dg
  - digestive gland
  - DNased RNA
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

This is a repeat of the previous qPCR from earlier today, BUT I think I might have used the wrong primers in the earlier qPCR (see below). Set up qPCR with the correct (I'm 100% sure of this) primers. [Plate layout/workup is here](https://genefishttp://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090610-02.jpg).

Results: Well, in retrospect it looks like I DID use the correct primers earlier! However, the problem is the same. But, the melting curves in the H2O-only samples don't seem to be the same as what is being seen in the RNA samples, suggesting that the signal in the H2O-only samples are likely primer dimers (melting curve peaks are shifted to the left and are VERY low signals; barely above background).

So, what to do now? Mac has a mad ea suggestion to spike some water with gDNA and DNase treat the sample to assess whether or not the Dase treatment is actually working or not. I think I'll do this.