---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-09 04:42:12+00:00
layout: post
slug: qpcr-re-dnased-abalone-dg-rna-from-earlier-today-3
title: qPCR - Re-DNased abalone Dg RNA from earlier today
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - Dg
  - digestive gland
  - DNased RNA
  - H.crach_16s_SYB_F/R
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

Done to verify removal of gDNA from RNA. Used H.crach_16s_syb_f/r primers. [PCR workup/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090608-01.jpg).

Results: Still f'ing gDNA! I'm pretty convinced that this is indeed due to the Ambion kit I'm using being old. Got mixed up with a newer kit, but neither had dates. Mac is going to be running a qPCR later today on DNased RNA that used the other Ambion kit. I will wait until the results of her qPCR to proceed.