---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-06-03 04:58:14+00:00
layout: post
slug: pcr-c-pugetti-dna-from-20090513-20090526
title: PCR - C.pugetti DNA from 20090513 & 20090526
categories:
  - 2009
  - Miscellaneous
tags:
  - Cycloclasticus pugetii
  - gDNA
  - gel
  - PCR
---

This is a repeat of yesterday's PCR with a fresh working stock in hopes of eliminating the source of contamination seen in the negative control yesterday.

![](https://eagle.fish.washington.edu/Arabidopsis/20090603.JPG)

Lane 1 - 100bp ladder

Lane 2 - 5/13 DNA

Lane 3 - 5/26 DNA

Lane 4 - Empty

Lane 5 - H2O

Results: Same problem as yesterday. Water sample is contaminated. This suggests that the primer stocks are contaminated. Likely due to the use of non-sterile, "old" TE for reconstitution of the primer stocks. Will re-order them and reconstitute them sterily.