---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-15 16:49:26+00:00
layout: post
slug: gdna-isolation-macs-bb-and-dh-site-samples
title: gDNA Isolation - Mac's BB and DH site samples
categories:
  - 2009
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DH
  - DNA Isolation
  - DNA Quantification
  - DNeasy
  - NanoDrop1000
  - Pacific oyster
---

Due to failure of gDNA isolation via the TriReagent method (see [20090511](https://robertslab.github.io/sams-notebook/posts/2009/2009-05-11-dna-isolation-macs-gigas-samples-from-20090505-20090506/)) used Qiagen DNeasy Kit. Digested samples for 3 hrs. at 55C in Proteinase K according to protocol. Performed on a subset of each site samples: BB#11-18 & DH#11-18.

![](https://eagle.fish.washington.edu/Arabidopsis/20090515%20gDNA%20SJW-01.bmp)

Results: Excellent yields and superb quality.