---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-15 16:51:44+00:00
layout: post
slug: gdna-isolation-gigas-dermo-samples
title: gDNA Isolation - Gigas Dermo Samples
categories:
  - 2009
  - Miscellaneous
tags:
  - Crassostrea virginica
  - Dermo
  - DNA Isolation
  - DNA Quantification
  - DNeasy
  - Eastern oyster
  - NanoDrop1000
  - Perkinsus marinus
---

Transferred tissue from previously Chelexed samples:

WP3, WP7, WP13, WP14, WP15, CY1 - These all tested negative for virginica gDNA (18s qPCR by Rony on DATE)

CY42, CY36 - These tested positive for virginica gDNA (18s qPCR by Rony on DATE).

Used Qiagen DNeasy Kit. Digested samples for 3 hrs. at 55C in Proteinase K according to protocol.

![](https://eagle.fish.washington.edu/Arabidopsis/20090515%20gDNA%20Dermo%20SJW-01.bmp)

Results: Horrible. Virtually no DNA in any of these samples. Additionally, the quality of the DNA is atrocious. Not sure what to do now.