---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-14 16:55:17+00:00
layout: post
slug: reverse-transcription-macs-gigas-dnased-rna-from-20090512
title: Reverse Transcription - Mac's gigas DNased RNA from 20090512
categories:
  - 2009
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DH
  - M-MLV
  - oligo dT
  - Pacific oyster
  - reverse transcription
  - RNA
---

Performed RT using Promega M-MLV RT according to M-MLV protocol and used 0.5ug oligo dT per ug of RNA on all BB and DH site samples that were negative for gDNA (see qPCR results [20090512](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090512)). [Calculations and work up are here](https://spreadsheets.google.com/ccc?key=0AmS_90rPaQMzdE1WT0dVbVFxQ0o1TkJXalF5MXFhcXc&hl=en). Samples were set up in a plate to facilitate sample loading in subsequent qPCRs.

UPDATE: cDNA plate was discarded 20120320 by SJW.
