---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-12 17:43:31+00:00
layout: post
slug: dnase-treatment-rigorous-macs-gigas-rnare-dnased-rna-from-20090507-20090508-respectively
title: DNase Treatment (Rigorous!) - Mac's gigas RNA/Re-DNased RNA from 20090507 &
  20090508, respectively
categories:
  - 2009
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DH
  - DNase
  - Pacific oyster
  - RNA
  - Turbo DNA-free
---

Followed the rigorous protocol for Ambion's Turbo DNA-free protocol for the following RNAs:

BB#11-20

DH#11-20

Used 10ug of RNA (200ng/uL) in 50uL as directed. Here are the calcs for FF and DH.

Followed standard protocol on DNased samples from [20090508](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090508):

BB#1-10

DH#1-10

The standard protocol should be fine for these samples, since the procedure worked on Friday for some of them.