---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-08 18:25:09+00:00
layout: post
slug: qpcr-re-dnased-oyster-rna-from-today
title: qPCR - Re-DNased oyster RNA from today
categories:
  - 2009
  - PROPS
tags:
  - Crassostrea gigas
  - DNased RNA
  - Gigas_18s_F/R
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO13
---

Performed qPCR on the re-DNased oyster RNA from earlier today with Gigas_18s_F/R primers to verify removal of detectable gDNA in the samples, since the initial qPCR from yesterday indicated residual gDNA was still present in the DNase treated RNA. [Plate layout/set up can be found here.](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090508-03.jpg)

Results: About 4 samples in each site set are NEGATIVE for gDNA. That means the remainder still have detectable levels of gDNA. Boo.