---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-08 18:23:17+00:00
layout: post
slug: dnase-treatment-oyster-rna-from-yesterday
title: DNase Treatment - Oyster RNA from yesterday
categories:
  - 2009
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DH
  - DNase
  - Pacific oyster
  - RNA
  - Turbo DNA-free
---

Yesterday's qPCR indicated that all of the RNA still contained gDNA contamination. So, took 10ug of RNA from BB #1-10 and DH#1-10 (calcs/workup [BB](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090508-01.jpg) and [DH](http://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090508-02.jpg)) and brought the volumes up to 50uL with 0.1% DEPC-H2O. Processed the samples according to Ambion Tubrbo DNA-free protocol. Will proceed with qPCR immediately.