---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-05-07 18:32:41+00:00
layout: post
slug: qpcr-dnased-oyster-rna-from-earlier-today
title: qPCR - DNased oyster RNA from earlier today
categories:
  - 2009
  - PROPS
tags:
  - 18s
  - BB
  - Crassostrea gigas
  - DH
  - DNased RNA
  - Gigas_18s_F/R
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO13
---

Performed qPCR on the DNased RNA to with Gigas_18s_F/R primers to verify removal of detectable gDNA in the samples. [Plate layout/set up can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090507-01.jpg).

Results: All samples produced a signal. In retrospect, this is likely due to having too much RNA for the DNase treatment. I proceeded with the DNase treatment and qPCR prior to specing the samples. Spec revealed that most of them were highly concentrated; more than the Ambion protocol recommends. Will redo the DNase treatment on a subset of the samples using the appropriate quantity of RNA.