---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-21 18:53:59+00:00
layout: post
slug: qpcr-abalone-dnased-rna-from-yesterday
title: qPCR - Abalone DNased RNA from yesterday
categories:
  - 2009
  - Miscellaneous
tags:
  - '08:3'
  - '08:4'
  - black abalone
  - Dg
  - digestive gland
  - DNased RNA
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

Performed qPCR to evaluate gDNA removal w/ 2x Immomx and SYTO 13. [qPCR/plate set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090421-01.jpg).

Results: The two cDNA samples come up as positive. No flourescence detected in any other gamples. However, melting curves look suspicous despite the fact that the "Quantitation" view indicates now amplification.
