--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-04-19 18:59:11+00:00 layout: post slug: bacteria-c-pugettii-culture title: Bacteria - C. pugettii culture categories: - 2009 - Miscellaneous tags: - ATCC - bacterial culture - biphenyl - Cycloclasticus pugetii - marine broth --- Rehydrated ATCC isolate according to directions. Added 5mL of 1x Marine Broth to a glass culture tube. Used 1mL of this to rehydrate ATCC sample and then added back into the culture tube. Added a few crystals of biphenyl, which had been exposed to UV for ~5mins prior. Incubated at 20C, no shaking. This was done at ~11:30AM.