---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-16 19:02:43+00:00
layout: post
slug: pcr-two-new-dungan-isolates
title: PCR - Two new Dungan isolates
categories:
  - 2009
  - Miscellaneous
tags:
  - AmpliTaq
  - Dungan isolates
  - gel
  - PCR
---

Repeat of yesterday's PCR, but with AmpliTaq, less gDNA and 50uL rxn volume. [PCR set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090416-01.jpg).

![](https://eagle.fish.washington.edu/Arabidopsis/20090416-02.JPG)

Gel loaded and run by Steven. Not entirely sure of the loading order. However, it doesn't really matter...

Results: Still absolutely nothing.



**UPDATE**: Noticed on 20090713 that the reactions didn't have dNTPs. Probably explains why it didn't work!