--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-04-15 19:31:44+00:00 layout: post slug: qpcr-rab7_sybr-primers-on-abalone-rna-and-dnased-rna title: qPCR - Rab7_SYBR primers on abalone RNA and DNased RNA categories: - 2009 - Miscellaneous tags: - black abalone - DNased RNA - Haliotis cracherodii - Immomix - Opticon2 - qPCR - Rab7_SYBR - RNA - SYTO13 --- Attempt to find out if gDNA contamination exists iafter Ambion treatment. Previous test (on [20090414](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090414)) suggests the QT Kit did not eliminate gDNA. [PCR set up and plate layout here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090415-02.jpg). Used Immomix and SYTO 13.