---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-15 19:20:41+00:00
layout: post
slug: pcr-two-new-dungan-isolates-from-earlier-today
title: PCR - Two new Dungan isolates from earlier today
categories:
  - 2009
  - Miscellaneous
tags:
  - Dungan isolates
  - Euk
  - gDNA
  - LABY
  - MIE-14y
  - PCR
  - VNTc-1.2-C1/G10
---

Set up PCRs on:

MIE-14y

VNTc-1.2-C1/G10

Used Euk A/B and LABY A/Y primers. Anneal temp 50C. PCR set up is here.

NOTE: Due to the extremely low concentrations of gDNA from these two samples, I used a large amount of gDNA in the rxns. Check the PCR set up link for actual numbers.

Lane 1 - Hyperladder (5uL)

Lane 2 - VNTc-1.2-C1/G10 (Euk primers)

Lane 3 - MIE-14y (Euk primers)

Lane 4 - H2O (Euk primers)

Lane 5 - H2O (Euk primers)

Lane 6 - VNTc-1.2-C1/G10 (Laby primers)

Lane 7 - MIE-14y (Laby primers)

Lane 8 - H2O (Laby primers)

Lane 9 - H2O (Laby primers)

lane 10 - 100bp Ladder

Results: Nada. Probably because of low [gDNA], but could also be due to PCR inhibitors in the gDNA. Will retry using Amplitaq and less gDNA.