---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-15 19:15:20+00:00
layout: post
slug: gdna-isolation-two-new-dungan-isolates
title: gDNA Isolation - Two new Dungan isolates
categories:
  - 2009
  - Miscellaneous
tags:
  - DNA Isolation
  - DNA Quantification
  - DNeasy
  - Dungan isolates
  - gDNA
  - MIE-14y
  - NanoDrop1000
  - VNTc-1.2-C1/G10
---

Isolated gDNA from the following two samples:

MIE-14y

VNTc-1.2-C1/G10

Samples were spun @ 16,000g @ RT for 2mins. No visible pellets in either sample. EtOH was removed. "Pellets" were washed in 1x PBS (pH=7.6) two times and then the Qiagen DNEasy Kit protocol was followed. Samples were incubated @ 55C with Proteinase K for ~2hrs.

![](https://eagle.fish.washington.edu/Arabidopsis/20090415%20gDNA%20SJW.jpg)

Results: Both samples show really, really low quantities of gDNA.