--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-04-14 19:34:06+00:00 layout: post slug: pcr-baysea-scallop-gdnas title: PCR - Bay/Sea scallop gDNAs categories: - 2009 - Miscellaneous tags: - actin - Argopecten irradians - bay scallop - gDNA - gel - PCR - Placopecten magellanicus - sea scallop --- Used higher annealing temps to improve primer specificity, compared to yesterday's results. [PCR set up and plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090414-02.png). ![](https://eagle.fish.washington.edu/Arabidopsis/20090414-01.JPG) See the PCR/plate set up link for samples. Hyperladder is placed between every 12 samples. Results: See this [Google Spreadsheet](https://spreadsheets.google.com/ccc?key=pwrSWt05L-jYWIFJ9BRI9Qg&hl=en) for a summary of the 4 gels from the last two days.