---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-13 23:04:34+00:00
layout: post
slug: pcr-baysea-scallop-gdna-isolated-earlier-today
title: PCR - Bay/Sea scallop gDNA isolated earlier today
categories:
  - 2009
  - Miscellaneous
tags:
  - actin
  - Argopecten irradians
  - bay scallop
  - gel
  - PCR
  - Placopecten magellanicus
  - sea scallop
---

Used 3 sets of reverse primers:

Bay_Actin_Rv0

Bay_Actin_Rv2

Sea_Actin_Rv2

Primers were slected based on information from Steven's notebook (#8, 12/30/2007-1/3/2008). Anneal temp 53C.

[PCR set up here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090413-01.jpg) . [Plate layout here](http://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090413-02.jpg) .

Samples were run out by Steven the following day.

Gel 1 of 3

![](https://eagle.fish.washington.edu/Arabidopsis/20090413-02.JPG)

Gel 2 of 3

![](https://eagle.fish.washington.edu/Arabidopsis/20090413-03.JPG)

Gel 3 of 3

![](https://eagle.fish.washington.edu/Arabidopsis/20090413-04.JPG)

Results: