---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-10 23:07:51+00:00
layout: post
slug: pcr-old-dungan-isolates-1-35-weukab-primers
title: 'PCR - Old Dungan isolates #1-35 w/EukA/B primers'
categories:
  - 2009
  - Miscellaneous
tags:
  - Dungan isolates
  - Euk
  - gel
  - PCR
---

Steven had me re-PCR the old Dungan isolates with the new EukA/B primers. Anneal temp 50C. [PCR set up here (bottom half of sheet)(https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090410-01.jpg) .

NOTE: Sample #30 was not in the rack of tubes that Steven gave to me.

![](https://eagle.fish.washington.edu/Arabidopsis/20090410-01.jpg)

Results: