---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-09 23:11:25+00:00
layout: post
slug: qpcr-repeat-modified-of-yesterdays-abalone-cdna-check
title: qPCR - Repeat (modified) of yesterday's abalone cDNA check
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - cDNA
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

qPCR was performed with 16s_sybr primers on a subset of the "No RT" cDNA rxns from yesterday at both 55C and 60C. [Sample set up and plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090409-01.jpg).

Results: Still getting signals in the "No RT" rxns and possibly in the waters. Cut run short to start another. Will test Lisa's previously DNased RNA.
