---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-09 23:12:58+00:00
layout: post
slug: qpcr-check-dnased-abalone-rna-by-lisa-for-gdna
title: qPCR - Check DNased abalone RNA (by Lisa) for gDNA
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - DNased RNA
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

qPCR was performed with 16s_sybr primers on the DNased RNA that Lisa did. Annel temp 55C. [Sample set up and plate layout is here.](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090409-02.jpg)

Results: Still got signals in all of the samples, including the waters. Personally, I think the primers are contaminated or are forming crazy dimers. Lisa came by and picked up cDNA to run other genes on.