---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-08 23:18:46+00:00
layout: post
slug: qpcr-abalone-cdna-qt-from-earlier-today
title: qPCR - Abalone cDNA (QT) from earlier today
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - cDNA
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - SYTO13
---

qPCR was performed with 16s_sybr primers on a subset of the cDNA rxns and all of the "No RT" rxns from earlier to detect the presence of contaminating gDNA. qPCR was also performed with the Rab7_sybr primers on a subset of the cDNA rxns to check that the assay would work. [The qPCR set up sheet/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090408-05.jpg). Annealing temp = 55C.

Results: Apparently the gDNA wipeout step did NOT work! Also, some signal in the water samples.
