---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-06 23:41:43+00:00
layout: post
slug: pcr-new-dungan-isolates
title: PCR - New Dungan isolates
categories:
  - 2009
  - Miscellaneous
tags:
  - 1.2t
  - 1.5t
  - 11t
  - 12t
  - 13t
  - 17t
  - 19t
  - AmpliTaq
  - Dungan isolates
  - gel
  - H5
  - PCR
---

Repeat of PCR from [20090403](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090403), but using AmpliTaq and 50C annealing temp. [PCR set up is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090406-01.jpg).

![](https://eagle.fish.washington.edu/Arabidopsis/20090407.JPG)

Lane 1 - Hyperladder

Lane 2 - 19t

Lane 3 - 100bp ladder

Lane 4 - 13t

Lane 6 - 17t

Lane 8 - 1.5t

Lane 9 - Hyperladder

Lane 10 - 1.2t

Lane 12 - 11t

Lane 13 - 100bp ladder

Lane 14 - H5

Lane 16 - 12t

Lane 17 - 100bp Ladder

Lane 18 - H2O

Lane 19 - H2O

Lane 20 - Hyperladder

Results: Nothing amplified! Possibly due to age of polymerase (?); over a year old. Will wait to repeat for new primers to arrive (EukA/B).
