---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-03 23:43:53+00:00
layout: post
slug: qpcr-abalone-rna-check-for-gdna
title: qPCR - Abalone RNA, check for gDNA
categories:
  - 2009
  - Miscellaneous
tags:
  - 16s
  - black abalone
  - Haliotis cracherodii
  - Immomix
  - Opticon2
  - qPCR
  - RNA
  - SYTO13
---

Abalone RNA isolated from this week was qPCR's with 16s primers to determine if gDNA contamination was present. [PCR/plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090403-01.pdf).

Results: All samples appear to have gDNA contamination. Will use Qiagen QT Kit for cDNA.
