---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-04-02 23:49:49+00:00
layout: post
slug: rna-isolation-abalone-digestive-gland-samples
title: RNA Isolation - Abalone digestive gland samples
categories:
  - 2009
  - Miscellaneous
tags:
  - '08:3'
  - '08:4'
  - black abalone
  - Dg
  - digestive gland
  - Haliotis cracherodii
  - NanoDrop1000
  - RNA isolation
  - RNA PowerSoil Kit
  - RNA quantification
---

Total RNA was isolated from the following abalone digestive gland samples using the RNA Powersoil Kit, according to their protocol:

08:3-17

08:3-18

08:4-2

08:4-3

08:4-4

08:4-5

08:4-7

08:4-8

08:4-11

Notes: 08:4-5 took hours to wash the column. 08:4-4 ended up with a dark RNA pellet and the reuspended RNA is discolored (brown-ish).

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090402%20RNA%20SJW.jpg)

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090403%20RNA%20SJW.jpg)

Results: All the RNA looks good except for 08:4-4, but that isn't surprising (see note above). Samples were stored @ -80C in the boxes from which the tissue samples came.
