--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-03-27 23:56:33+00:00 layout: post slug: rna-isolation-abalone-digestive-gland-samples-3 title: RNA Isolation - Abalone digestive gland samples categories: - 2009 - Miscellaneous tags: - '08:3' - black abalone - Dg - digestive gland - Haliotis cracherodii - NanoDrop1000 - RNA - RNA isolation - RNA PowerSoil Kit - RNA quantification --- Total RNA was isolated from the following abalone digestive gland samples using the RNA Powersoil Kit, according to their protocol: 08:3-11 08:3-12 08:3-13 08:3-19 08:3-20 08:3-21 08:3-22 08:3-23 Notes: After phase separation, 3-11 and 3-12 had milky/cloudy aqueous phases. These two samples were subjected to another 10min spin @ 2500g, but this spin made no difference in their appearance. Other samples were clear or slightly translucent at worst. ![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090330%20RNA%20SJW.jpg) Results: RNA looks great in nearly all of the samples. RNA has been stored @ -80C in the same box from where the tissue was taken.