---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-03-03 01:10:17+00:00
layout: post
slug: qpcr-repeat-of-qpcr-from-earlier-today-with-fresh-primer-working-stocks
title: qPCR - Repeat of qPCR from earlier today with fresh primer working stocks
categories:
  - 2009
  - Miscellaneous
tags:
  - '16s'
  - Opticon2
  - qPCR
  - SensiMix
  - SYBR
  - Vibrio tubiashii
  - Vtub_16s_V3
---

This is an exact repeat of the qPCR from earlier today, but using a fresh working stock of the Vtub_16s_V3 primers. [The plate layout/qPCR workup is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090302-2.jpg).

Results: Same as earlier today. Must be a bacterial contaminant somehwere that these 16s primers are picking up. Will order IGS primers that are species specific found in Lee et al. 2002.
