---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-02-25 02:05:48+00:00
layout: post
slug: reverse-transcription-v-tubiashii-dnased-rna-from-yesterday
title: Reverse Transcription - V.tubiashii DNAsed RNA (from yesterday)
categories:
  - 2009
  - Miscellaneous
tags:
  - cDNA
  - DNased RNA
  - M-MLV
  - random primers
  - reverse transcription
  - Vibrio tubiashii
---

Set up the MMLV RT rxns with random primers using ~833ng DNAsed RNA (prepared yesterday) according to the [Promega MMLV Product Insert](https://aquacul4.fish.washington.edu/Protocols:Information%20Sheets/Product%20Information%20Sheets/Promega%20-%20MMLV%20RT.pdf). This procedure is slightly different than what is in our lab protocol for RT rxns. [Here is the workup for the rxns](http://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090224-1.jpg). cDNA was stored @ -20C in the "Vibrio" box.