---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-02-24 02:09:38+00:00
layout: post
slug: rna-isolation-v-tubiashii-samples-from-autoclaved-gigas-exposure-from-20081218
title: RNA Isolation - V.tubiashii samples from autoclaved gigas exposure (from 20081218)
categories:
  - 2009
  - Miscellaneous
tags:
  - DNase
  - NanoDrop1000
  - RNA
  - RNA isolation
  - RNA quantification
  - TriReagent
  - Turbo DNA-free
  - Vibrio tubiashii
---

RNA was isolated from the Control and V.tub+gigas samples from the 0, 1, & 24hr time points using 1mL TriReagent. No visible pellets. Used 20uL of 0.1%DEPC-H2O to resuspend RNA. Incubated @ 55C, 5mins. Spec'd.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090223%20RNA%20SJW.jpg)

Results: RNA looks OK, but not great. For the "V.tub + gigas t=1" sample, the third spec reading is correct. The first two had the air bubble error.





### DNAse Treatment - V.tubiashii total RNA (see above)



1ug of RNA in a volume of 12uL was DNAsed using the Ambion DNA-free Kit according to their protocol. RNA was transferred to a fresh tube and stored @ -80C in Sam's RNA Box #1.