---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-23 02:59:13+00:00
layout: post
slug: rna-hard-clam-hemo-rna-from-20090121
title: RNA - Hard clam hemo RNA (from 20090121)
categories:
  - 2009
  - Miscellaneous
tags:
  - Hard clam
  - hemocyte
  - Mercenaria mercenaria
  - mRNA
  - mRNA enrichment
---

The two RNA samples from yesterday were precipitated and washed according to the [Ambion PolyA Purist protocol](https://aquacul4.fish.washington.edu/Protocols:Information%20Sheets/Commercial%20Protocols:Manuals/Ambion%20-%20MicroPoly%28A%29Purist%20Kit.pdf) and resuspended in 50uL of 0.1% DEPC-H2O.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090122%20RNA%20SJW.png)

Results: RNA readings look better than they did prior to precipitation. The hemo RNA samples will be combined with previous hemo RNA samples and mRNA will be isolated using the Ambion PolyA Purist Kit.