---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-21 03:15:09+00:00
layout: post
slug: rna-precipitation-of-hard-clam-hemo-rna-from-20090116
title: RNA - Precipitation of Hard Clam Hemo RNA from 20090116
categories:
  - 2009
  - Miscellaneous
tags:
  - EtOH precipitation
  - gill
  - Hard clam
  - hemocyte
  - Mercenaria mercenaria
  - NanoDrop1000
  - RNA
  - RNA quantification
---

RNA isolated on 20090116 was precipitated over the weekend @ -20C. Samples were treated according to [Ambion PolyA Purist protocol](https://aquacul4.fish.washington.edu/Protocols:Information%20Sheets/Commercial%20Protocols:Manuals/Ambion%20-%20MicroPoly%28A%29Purist%20Kit.pdf) and resuspended in 100uL of 0.1% DEPC-H2O. Samples were stored in the red "hard clam" box @ -80C.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090120%20RNA%20SJW.png)

Results: 260/280 look good for both gill and hemo samples. 260/230 looks OK for gill, but looks horrible (as usual) for hemos.